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| Registros recuperados : 13 | |
| 6. |  | BENDAHAN, A. B.; TOURRAND, J. F.; MOURÃO, M. DA VEIGA, J. B.; BOMMEL, P. POCCARD CHAPUIS, R. Reducing the deforestation of the Amazon through farming system intensification. In: EUROPEAN IFSA SYMPOSIUM, 8., 2008, France. Paris: INRA, 2008.| Biblioteca(s): Embrapa Roraima. |
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| 7. | | CIALDELLA, N.; CARVALHO, S.; VAZ, V.; BARBOSA, T.; THÂLES, M.; MOURÃO, M.; POCCARD-CHAPUIS, R.; TOURRAND, J.-F. Do political changes aimed at reducing Amazonian deforestation contribute to ecological intensification? Cahiers Agricultures, v. 24, n. 84, p. 246-254, juillet-aout 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | XAVIER JUNIOR, S. R.; MARTINS-DA-SILVA, R. C. V.; ILKIU-BORGES, F.; MOURÃO, M.; RUSCHELL, A.; CRUZ, E.; SARAIVA, G. de S. Levantamento florístico preliminar de uma área de floresta ripária na mesobacia do igarapé Timboteua, Pará, Brasil. In: SEMINÁRIO GESTABACIAS, 1., 2010, Belém, PA. Resultados de pesquisas em mesobacias do Nordeste paraense e sua aplicação no desenvolvimento rural. Belém, PA: Embrapa Amazônia Oriental: Projeto Gestabacias, 2010. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Shistosoma mansoni. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. Não paginado. X-MEETING 2011.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 10. | | CARVALHO, C. J. R. de; FREITAS, J. F. B. de; MOURÃO, M.; MAIA, R.; SOUZA, C. M.; SILVA, T. P. da; TRINDADE, I. A.; FREITAS, F. S.; SAMPAIO, I. C. G.; SERRÃO, B. de O.; VASCONCELOS, S. S. Avaliação de indicadores biogeoquímicos em diferentes sistemas de uso da terra na Amazônia Oriental. In: CONFERÊNCIA DO SUBPROGRAMA DE CIÊNCIA E TECNOLOGIA - SPC&T FASE II/PPG7, 2008, Belém, PA. Anais... Brasília, DF: CNPq, 2009. p. 441-445. Disponível também em CD-ROM (CD 00173).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 12. | | KATO, O. R.; FREITAS, A. C. R. de; FERREIRA, J. H.; LEMOS, W. P.; GONÇALVES, R. C.; RODRIGUES FILHO, J. A.; AZEVEDO, C. M. B. C. de; VASCONCELOS, M. A.; MOURÃO, M.; MATOS, L. M. S. de; GATO, R. F.; SHIMIZU, M.; ROFFE, A. Recuperação de área degradada através do preparo de área sem queima e sistemas agroflorestais. In: CONFERÊNCIA DO SUBPROGRAMA DE CIÊNCIA E TECNOLOGIA - SPC&T FASE II/PPG7, 2008, Belém, PA. Anais... Brasília, DF: CNPq, 2009. p. 494-499. Disponível também em CD-ROM (CD 00173).| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| 13. | | ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. Molecular studies of the brazilian infectious bronchitis virus isolates. Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 13 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
10/02/2014 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. |
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Afiliação: |
MARINA DE MORAES MOURÃO, Centro de Pesquisas René Rachou-Fiocruz; MAINÁ BITAR, UFMG; FRANCISCO PEREIRA LOBO, CNPTIA; ANA PAULA PECONICK, Ufla; PRISCILA GRYNBERG, UFMG; FRANCISCO PROSDOCIMI, UFRJ; MICHAEL WAISBERG, University of Virginia; GUSTAVO COUTINHO CERQUEIRA, Broad Institute of Massachusetts Institute of Technology and Harvard; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; TIMOTHY YOSHINO, University of Wisconsin; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013. |
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Idioma: |
Inglês |
|
Conteúdo: |
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974). MenosSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different librari... Mostrar Tudo |
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Palavras-Chave: |
Interferência de RNA. |
|
Thesagro: |
Schistosoma Mansoni. |
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Thesaurus NAL: |
RNA interference; Trans-splicing. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02851naa a2200301 a 4500
001 1979292
005 2020-01-08
008 2013 bl uuuu u00u1 u #d
100 1 $aMOURÃO, M. de M.
245 $aA directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni.$h[electronic resource]
260 $c2013
520 $aSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
650 $aRNA interference
650 $aTrans-splicing
650 $aSchistosoma Mansoni
653 $aInterferência de RNA
700 1 $aBITAR, M.
700 1 $aLOBO, F. P.
700 1 $aPECONICK, A. P.
700 1 $aGRYNBERG, P.
700 1 $aPROSDOCIMI, F.
700 1 $aWAISBERG, M.
700 1 $aCERQUEIRA, G. C.
700 1 $aMACEDO, A. M.
700 1 $aMACHADO, C. R.
700 1 $aYOSHINO, T.
700 1 $aFRANCO, G. R.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 108, n. 6, p. 707-717, Sept. 2013.
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